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. 2014 Sep 4;289(42):29446–29456. doi: 10.1074/jbc.M114.578492

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FIGURE 10. — Confirmation of STIM1/Orai1-mediated nuclear import of NFAT by fractionation assays. Representative Western blot data following fractionation assays indicate a complete separation of cytoplasmic (Cyto) and nuclear (Nuc) contents from HUVECs expressing eGFP-NFATc1. The presence of eGFP-NFATc1 in the cytoplasmic and/or nuclear extracts under six different conditions was assessed by anti-GFP blotting. MW: molecular weight; CsA: cyclosporin A; KD: knocking-down; HDAC1: a nuclear marker; α-tubulin: a cytoplasmic marker.