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CRAC channel blockers eliminate the sustained component of histamine-evoked calcium mobilization. A–C, representative [Ca2+]i traces show histamine-evoked [Ca2+]i mobilization from control HUVECs (A, n = 10 cells) or cells treated with 10 μm Gd3+ (B, n = 33 cells) or 10 μm BTP2 (C, n = 30 cells) before the application of 100 μm histamine. D, averaged value of sustained [Ca2+]i was obtained at 4 min after histamine application. *: p < 0.01 compared with control.
