FIGURE 5.
Silencing of the STIM1 gene abolishes both thapsigargin- and histamine-evoked calcium entries in HUVECs. A–C, representative [Ca2+]i recordings show TG-triggered SOCE in HUVECs treated with vehicle control (A, n = 25 cells), non-targeting siRNA (B, n = 28 cells), or siRNA against STIM1 (C, n = 25 cells). D–F, representative [Ca2+]i responses to histamine from cells treated with vehicle control (D, n = 41 cells), non-targeting siRNA (E, n = 31 cells), or STIM1-siRNA (F, n = 51 cells). G–H, averaged peak (G) and sustained (H) values of [Ca2+]i for TG-evoked SOCE and histamine-induced Ca2+ entry, respectively, were compared among the treatments above. *: p < 0.01 compared with control. I, Western blot validation of siRNA-mediated gene silencing of STIM1 in HUVECs is shown. The protein levels of GAPDH from individual samples were examined to verify the application of equal amount of total cell lysates for Western blot assays. MW: molecular weight; Ctl: control; NT: non-targeting.