Fig. 4.

Effects of ANG II and CAV-1 siRNA alone or in combination on ANG II-induced phosphorylation of Na⁺/H⁺ exchanger 3 (NHE3) in mPCT cells. mPCT cells pretransfected with or without CAV-1 siRNA were incubated with ANG II (10 nM) at 37°C for 1 h before proteins were extracted for Western blots of total and phosphorylated NHE3 proteins. **P < 0.01 vs. control mPCT cells not transfected with CAV-1 siRNAs; ++P < 0.01 vs. ANG II-treated mPCT cells.