Fig. 2.

Loss of sEH prevents interstitial fibrogenesis induced by UUO. A: collagen deposition detected by Sirius red stain and α-smooth muscle actin (α-SMA) expression stained with α-SMA monoclonal antibody on kidney sections in WT or sEH-KO mice at 10 days after sham operation or UUO. Arrow heads indicate glomeruli. Scale bars = 50 μm. To demonstrate the specific staining of α-SMA in the myofibroblasts in the interstitial area, representative high magnification of the α-SMA staining regions from UUO-induced kidneys is shown in the bottom panels. B: percent of Sirius red-positive area on kidney sections. C: collagen content represented by hydroxyproline level in the kidneys. D: percent of α-SMA-positive area on the kidney sections. E: profibrotic expression of α-SMA and fibronectin in the kidneys evaluated by Western blot analyses. The bands were quantified using Lab Works analysis software. Error bars represent SD (n = 5). *P < 0.05 vs. sham. #P < 0.05 vs. WT. §P < 0.05 vs. 3 days.