Fig. 1.

Functional overload induces Ca²⁺/calmodulin-dependent protein kinase kinase-α (CaMKKα) protein expression in a time-dependent manner in mouse skeletal muscle. Mouse plantaris muscles were stimulated to hypertrophy via unilateral functional overload. After 1, 3, 7, or 10 days, muscles were frozen, weighed, and processed for immunoblot analyses. A: muscle frozen wet weights. B: representative blots and quantification for CaMKKα; n = 7–8 muscles/group. P < 0.05 = * vs. sham, # vs. 1 day, $ vs. 3 days, % vs. 7 days.