Fig. 2.

Expression of constitutively active CaMKKα (CA-CAMKKα) increases skeletal muscle mass and protein content. Mouse extensor digitorum longus muscles were transfected with empty vector or constitutively active CaMKKα using in vivo electroporation. Mice were euthanized 2 wk later and muscle collected. A: muscle wet weight. B: muscles were dehydrated for 8 h and reweighed (i.e., dry weight). C: percent loss of muscle weight with dehydration was calculated and is presented as percent muscle water content. D: dehydrated muscles were homogenized and muscle protein content determined; n = 8 muscles/group. P < 0.05 = * vs. empty vector.