Fig. 1.
Sentrin/SUMO-specific protease-1 (SENP1) induces secretory dysfunction in islets. SUMO, small ubiquitin-like modifier. A: SENP1 tagged to GFP in mouse islets and the loading control β-actin. B: insulin secretion normalized to protein content from mouse islets transduced with Ad-GFP (open bars) or Ad-GFP-SENP1 (filled bars). C: KCl-stimulated insulin secretion from mouse islets transduced with Ad-GFP (○) or Ad-SENP1 (●), normalized to baseline insulin secretion. Area under the curve (AUC) of the secretory response is shown (right). D: responses in [Ca2+]i from mouse islets transduced with Ad-GFP or Ad-SENP1 following glucose-stimulation (arrow). E: glucose-stimulated [Ca2+]i (GSCa) responses. F: amplitude of [Ca2+]i oscillations in mouse pancreatic islets. *P < 0.05.