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. 2014 Aug 26;307(8):E664–E673. doi: 10.1152/ajpendo.00168.2014

Fig. 3.

Fig. 3.

SENP1 induces endoplasmic reticulum (ER) stress and enhances IL-1β-induced transcription of NF-κB target genes. A and B: INS-1 832/13 cells were transduced with Ad-GFP or Ad-GFP-SENP1. RT-PCR detection and quantification of C/EB homologous protein (CHOP; A) or activating transcription factor 4 (ATF4; B) mRNA expression, normalized to β-actin mRNA, was determined. Levels of mRNA expression as %Ad-GFP control cells are shown. C: proinsulin secretion as %insulin secretion at 2.8 mmol/l glucose from mouse islets transduced with Ad-GFP or Ad-GFP-SENP1. D: INS-1 832/13 cells transduced with Ad-GFP or Ad-GFP-SENP1 were treated with IL-1β (10 ng/ml) for 6 h. RT-PCR detection and quantification of Nos2 mRNA expression, normalized to β-actin mRNA, was determined. Levels of Nos2 as %IL-1β-treated control cells are shown. E: immunoblotting for iNOS and β-actin (left) from INS-1 832/13 cells transduced with Ad-GFP or Ad-GFP-SENP1 and treated with IL-1β for 24 h. Quantification by densitometry relative to IL-1β-treated control cells is shown (right). F: nitrite accumulation in culture medium normalized to protein content of INS-1 832/13 cells transduced with Ad-GFP or Ad-GFP-SENP1 and treated with IL-1β (10 ng/ml) for 24 h. *P < 0.05, ***P < 0.005.