Table 3.
Gene microarray comparison of ImChief and ImSPEM cells
| Gene Symbol | ImChief Expression Level | ImSPEM Expression Level | Fold Change |
|---|---|---|---|
| Ppbp | 5.91 | 13.85 | 244.85 |
| Krt5 | 6.81 | 13.80 | 127.20 |
| Mal2 | 5.85 | 12.68 | 113.25 |
| Krt6a | 6.61 | 13.17 | 94.35 |
| Trim29 | 6.60 | 13.07 | 89.06 |
| Cldn4 | 7.28 | 13.48 | 73.53 |
| Dsg3 | 5.81 | 11.99 | 72.20 |
| Spink5 | 6.18 | 12.32 | 70.83 |
| Rab25 | 6.76 | 12.77 | 64.46 |
| Vsnl1 | 6.79 | 12.77 | 63.39 |
| Krt14 | 7.57 | 13.50 | 61.07 |
| Krt17 | 7.60 | 13.53 | 60.73 |
| Tacstd2 | 6.68 | 12.58 | 59.53 |
| Serpinb5 | 5.84 | 11.70 | 57.83 |
| Il1a | 5.29 | 11.13 | 57.20 |
| Xist | 5.87 | 11.71 | 57.18 |
| Dsp | 6.64 | 12.47 | 57.00 |
| Cdh1 | 6.76 | 12.44 | 51.21 |
| Cdh3 | 6.67 | 12.26 | 48.17 |
| Trex2 | 6.81 | 12.32 | 45.36 |
| Mpzl2 | 6.47 | 11.97 | 45.34 |
| Plet1 | 7.35 | 12.85 | 45.01 |
| Rab15 | 6.46 | 11.89 | 43.22 |
| Clic3 | 7.01 | 12.41 | 42.06 |
| Gpx2 | 6.53 | 11.90 | 41.43 |
| S100a14 | 6.58 | 11.93 | 40.85 |
| Nipal2 | 5.60 | 10.91 | 39.73 |
| Tspan6 | 6.37 | 11.67 | 39.54 |
| Krt6b | 6.11 | 11.31 | 36.67 |
| Cwh43 | 6.42 | 11.55 | 35.20 |
| Krt15 | 7.13 | 12.25 | 34.85 |
| Krt7 | 8.19 | 13.28 | 34.09 |
| Gabrp | 6.46 | 11.55 | 34.05 |
| Ehf | 6.55 | 11.57 | 32.46 |
| Wfdc2/He4 | 7.01 | 12.01 | 31.89 |
| Vnn1 | 7.37 | 12.35 | 31.54 |
| Esrp1 | 5.90 | 10.86 | 31.13 |
| Tmc4 | 7.14 | 12.07 | 30.57 |
| Gsta4 | 8.35 | 13.27 | 30.25 |
| Lass3 | 6.04 | 10.91 | 29.17 |
| Cxcl5 | 6.02 | 10.87 | 28.77 |
| Serinc2 | 7.97 | 12.79 | 28.19 |
| Tns4 | 6.70 | 11.47 | 27.41 |
| Ap1m2 | 6.19 | 10.95 | 27.05 |
| Irf6 | 6.54 | 11.28 | 26.83 |
| Elf3 | 6.38 | 11.10 | 26.40 |
| Sncg | 7.57 | 12.29 | 26.33 |
| Fgfbp1 | 6.43 | 11.15 | 26.25 |
| Tmprss11e | 5.81 | 10.51 | 26.09 |
| Pstpip2 F830208 | |||
| F22Rik | 5.71 | 10.38 | 25.43 |
ImChief cells and ImSPEM cells were grown at the nonpermissive temperature (39°C) for 1 wk. Transcriptional expression was analyzed by gene microarray. The top 50 transcripts upregulated in ImSPEM cells compared with ImChief cells reveal putative new markers of SPEM. The normalized expression level (in log base 2) in the respective cell line is shown as well as the linear fold change (converted to linear from log base 2).