Table 4.

Comparison of upregulated transcripts in ImSPEM with previously reported upregulated SPEM transcripts

Gene Symbol	ImChief Expression Level	ImSPEM Expression Level	Fold Change
Gpx2	6.53	11.90	41.43
Wfdc2/He4	7.01	12.01	31.89
Thbs1	9.23	13.45	18.62
Urah	7.12	10.92	13.89
Clu	9.54	12.19	6.27
Cd14	7.62	10.06	5.42
Cd9	9.60	11.96	5.13
Bzw2	8.97	11.01	4.11
Myc	10.59	12.22	3.10
Memo1	8.08	9.28	2.29
Mgst1	9.28	10.45	2.25
Psma3	10.18	11.35	2.24
Yars2	8.67	9.80	2.19
Mphosph6	8.16	9.26	2.15

The list of upregulated transcripts in ImSPEM cells compared with ImChief cells was compared with a previously reported list of transcripts upregulated in laser captured microdissected murine L635-induced SPEM lineages compared with normal chief cells (63). This comparison revealed 14 shared upregulated transcripts. The normalized expression levels in the ImSPEM and ImChief cell lines are shown as well as the fold change. The normalized expression levels of these cell lines could not be directly compared with the normalized expression levels of the tissue isolated chief cells and SPEM because of differences in RNA isolation and amplification methods.