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. 2014 Nov;28(11):4583–4590. doi: 10.1096/fj.13-243733

Table 1.

Culture conditions kept constant across all geometries during conditioned medium collection

Cell type Cells (n/μl)
Gel:medium ratio Average surface area per cell
Medium Hydrogel Lumen Well plate
HUVECs ∼800 ∼2,000 0.375 2.5 × 10−6 cm2 2.3 × 10−6 cm2
iPSC-ECs ∼550 ∼1,500 0.375 3.6 × 10−6 cm2 2.7 × 10−6 cm2
Primary endothelial cells ∼1700 ∼4,200 0.375 1.2 × 10−6 cm2 0.9 × 10−6 cm2
MCF10a cells ∼3750 ∼10,000 0.375 0.5 × 10 −6 cm2 0.5 × 10−6 cm2
HMEC-15-htert cells ∼3750 ∼10,000 0.375 0.5 × 10−6cm2 0.4 × 10−6 cm2
HMEC-SR cells ∼3750 ∼10,000 0.375 0.5 × 10−6cm2 0.4 × 10−6 cm2

HUVECs and MCF10a cells were set up in 48-well plates, all other types in 384-well plates.