Table 1.
Culture conditions kept constant across all geometries during conditioned medium collection
| Cell type | Cells (n/μl) |
Gel:medium ratio | Average surface area per cell |
||
|---|---|---|---|---|---|
| Medium | Hydrogel | Lumen | Well plate | ||
| HUVECs | ∼800 | ∼2,000 | 0.375 | 2.5 × 10−6 cm2 | 2.3 × 10−6 cm2 |
| iPSC-ECs | ∼550 | ∼1,500 | 0.375 | 3.6 × 10−6 cm2 | 2.7 × 10−6 cm2 |
| Primary endothelial cells | ∼1700 | ∼4,200 | 0.375 | 1.2 × 10−6 cm2 | 0.9 × 10−6 cm2 |
| MCF10a cells | ∼3750 | ∼10,000 | 0.375 | 0.5 × 10 −6 cm2 | 0.5 × 10−6 cm2 |
| HMEC-15-htert cells | ∼3750 | ∼10,000 | 0.375 | 0.5 × 10−6cm2 | 0.4 × 10−6 cm2 |
| HMEC-SR cells | ∼3750 | ∼10,000 | 0.375 | 0.5 × 10−6cm2 | 0.4 × 10−6 cm2 |
HUVECs and MCF10a cells were set up in 48-well plates, all other types in 384-well plates.