Fig. 7.

Targeted disruption of PbIMC1c and PbIMC1e. a Gene structure of pbimc1 alleles in WT and PbIMC1-KO parasite lines. The pbimc1 gene is indicated with coding sequence (wide bars) and non-coding sequence (narrow bars). Also indicated are the enhanced GFP module (gfp) and the hDHFR selectable marker gene cassette (hdhfr). b Confocal bright-field and GFP fluorescence images of gametocytes after attempted disruption of pbimc1c (top panel) and pbimc1e (bottom panel). Hoechst DNA staining (blue) labels nuclei