Table 1.
Primer | Sequence (5′-3′) |
---|---|
F3 | TTCCCTCTTCTGCCCTCC |
B3 | TCCTGTTCGGGATCCTCG |
FIP | GTAAACCGTCCCCGGCGATGTTTTGGGCATCTTCCCTGCATTG |
BIP | CTTTGTCCTGTTGGCCAGGCTCTTTTGACGAGCATAAAGCCTCTCC |
LF | TACACGGCTCGGTAACAGGA |
LB | CCACATCCGGCTCCGGAGCC |
TTTT is a thymidine linker in the FIP and BIP primers
The primers were designed based on the meq gene sequence of the RB1B very virulent plus MDV strain (GenBank accession number AY571783), using Primer Explorer version 4 (NetLaboratory, Tokyo, Japan)