Table 2.
Detection of MDV DNA in dust samples by LAMP
| Sample | DNA purity A260/A280 ratio/concentration (ng/µL) | Ct value LAMP MDV-1 | Melting temperature |
|---|---|---|---|
| Negative control | 1.83/125.0 | 40.0 | - |
| Farm 1 | 0.47/24.3 | 30.4 | 87.4 |
| Farm 2 | 0.38/15.9 | 18.3 | 86.9 |
| Farm 3 | 0.41/19.0 | 21.4 | 86.8 |
| Farm 4 | 0.42/20.5 | 15.4 | 87.4 |
| Farm 5 | 0.45/27.5 | 40.0 | - |
| Farm 6 | 0.41/19.9 | 16.7 | 86.9 |
| Farm 7 | 0.45/20.1 | 20.5 | 87.4 |
| Farm 8 | 0.41/19.4 | 20.7 | 87.3 |
| Farm 9 | 0.39/7.9 | 14.0 | 86.5 |
| Farm 10 | 1.0/12.7 | 20.9 | 86.8 |
| Farm 11 | 0.46/18.4 | 17.0 | 87.1 |
| Farm 12 | 0.40/10.5 | 15.9 | 86.3 |
| Positive control DNA of 31/07 MDV strain | 1.85/200.5 | 11.8 | 87.4 |
The measured DNA purity ratio (A260/A280), concentration (ng/µL), cycle threshold value and melting temperature of LAMP products are given. Negative control, DNA extracted from uninfected SPF chicken embryo fibroblasts (CEFs)