Table 3.

Mean response factors (f _a) and determination coefficients (R ²) obtained for phycobilin standards (ProZyme, Inc.) dilution series

Spectrophotometer
Symbol of phycobilin	λ max (nm)	Number of data (n)	Response factor f a μg cm−3 · [abs]−1		Determination coefficient (R 2)	Detection limit (μg cm−3)
			Symbol	Value
PC	620	18	f a,PC,abs	1.83E + 02	0.999	1.00
APC	650	18	f a,APC,abs	2.36E + 02	0.994	1.00
PE	565	18	f a,PE,abs	1.27E + 02	0.999	1.00
Fluorescence spectroscopy
Symbol of phycobilin	Lamp intensity (V)	Number of data (n)	Response factor f a μg cm−3 · [a.u.]−1		Determination coefficient (R 2)	Detection limit (μg cm−3)
			Symbol	Value
PC λ ex = 590 nm λ em = 644 nm	600	53	f a,PC,600	1.521E − 02	0.994	0.0075
	800	52	f a,PC,800	1.170E − 03	0.998	0.0075
	1000	42	f a,PC,1,000	1.895E − 04	0.985	0.001
APC λ ex = 600 nm λ em = 660 nm	600	34	f a,APC,600	1.991E − 02	0.991	0.01
	800	34	f a,APC,800	1.569E − 03	0.989	0.01
	1000	25	f a,APC,1,000	3.318E − 04	0.994	0.005
PE λ ex = 530 nm λ em = 576 nm	600	47	f a,PE,600	2.307E − 03	0.998	0.001
	800	31	f a,PE,800	1.671E − 04	0.998	0.001
	1000	20	f a,PE,1,000	2.808E − 05	0.997	0.001