Fig. 2.

RT-PCR of pectin acetylesterase T-DNA insertion lines. RT-PCR for PAE3 transcript (pae3-1, SALK_066524C; pae3-2, SALK_137505C; 3-week-old leaves); PAE5 (pae5-1, SALK_140555; pae5-2, SALK_052303C; 3-week-old leaves); PAE6 (pae6-1, SALK_020618; pae6-2, SALK_134907; 19-day-old leaves); PAE8 (pae8, SALK_132026; 2-week-old leaves); PAE9 (pae9-1, SALK_046973C; pae9-2, GABI-803G08; 3-week-old leaves); PAE7 (pae7-1, SALK_093502C; pae7-2, GABI_272B08, 19-day-old leaves); PAE10 (pae10-1, SALK_043807; pae10-2, SAIL_802_C05; 3-week-old leaves); PAE11 (pae11-1, SALK_049340.48.65.x; pae11-2, GABI_505H02; 19-day-old leaves); PAE12 (pae12-1, GABI_018A02; pae12-2, GABI_646F06; 3-week-old leaves). PTB—housekeeping gene. WT = Col-0 plants of same age as insertion lines being tested. Primers used for each reaction are listed in Suppl. Table S2