Table 3.
Galacturonic acid content of cell wall, pectin extract, residue and fractions in pae8, pae9 and double mutant (mg g−1 cell wall)
| WT | pae9-1 | pae9-2 | pae9-1/ox9-1 | pae9-1/ox9-2 | pae9-1/ox9-3 | pae8 | pae8/comp-1 | pae8/comp-2 | pae8/comp-3 | pae8/pae9-2 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell wall | 180.4 ± 12.2 | 171.8 ± 4.0 | 181.2 ± 3.7 | 191.2 ± 3.9 | 176.0 ± 7.5 | 175.0 ± 9.5 | 179.7 ± 10.2 | 175.9 ± 5.1 | 181.0 ± 5.5 | 184.7 ± 4.9 | 169.1 ± 2.6 |
| Pectin extract | 192.0 ± 22.9 | 192.0 ± 0.9 | 229.2 ± 60.8 | 205.2 ± 1.0 | 179.0 ± 11.3 | 153.4 ± 40.2 | 171.0 ± 8.5 | 157.5 ± 14.3 | 155.6 ± 4.7 | 172.2 ± 9.5 | 169.6 ± 6.3 |
| Pectin residue | 38.9 ± 6.1 | 44.0 ± 2.3 | 44.7 ± 2.6 | 47.5 ± 3.2 | 44.4 ± 3.9 | 42.0 ± 4.7 | 36.3 ± 5.1 | 31.3 ± 4.4 | 32.5 ± 1.2 | 34.5 ± 1.4 | 42.7 ± 1.1 |
| Fraction I | 5.7 ± 0.7 | 6.3 ± 0.7 | 5.3 ± 0.8 | 7.2* ± 0.3 | 5.0 ± 0.5 | 5.3 ± 0.1 | 4.5 ± 0.4 | ||||
| Fraction II | 6.5 ± 0.7 | 5.7 ± 0.2 | 5.3* ± 0.3 | 7.0 ± 1.6 | 6.2 ± 0.9 | 6.9 ± 0.7 | 4.6* ± 0.1 | ||||
| Fraction III | 16.6 ± 1.6 | 16.8 ± 1.0 | 16.4 ± 1.1 | 18.0 ± 0.2 | 16.5 ± 0.6 | 15.7 ± 0.8 | 16.4 ± 0.6 | ||||
| Fraction IV | 43.0 ± 0.9 | 41.7 ± 1.9 | 45.0 ± 4.9 | 43.7 ± 0.9 | 44.1 ± 1.9 | 40.8 ± 2.3 | 45.3 ± 1.1 | ||||
| Fraction V | 65.6 ± 5.9 | 62.3 ± 11.8 | 59.0 ± 9.90 | 67.7 ± 5.4 | 64.0 ± 4.4 | 68.3 ± 11.2 | 56.3 ± 3.2 |
The entire monosaccharide composition of these fractions can be found in Figs. S2, S3 and S4
ox9 overexpression of the PAE9 coding sequence, comp complementation with the native promoter and genomic sequence of PAE8, WT Col-0
± Indicates standard deviation
* Statistical significant differences from WT based t test (P < 0.01); n ≥ 3