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A: immunoreactivity for total tyrosine phosphorylated proteins (4G10) in total lysate (20 μg protein) after treatment with ANG II (100 nM). B: representative Western blot analysis for Src kinase in total lysate after ANG II (100 nM) treatment. Densitometric analysis was performed after normalizing Src kinase immunoreactivity with GAPDH. *Significantly different from control (P < 0.05, Student's t-test).
