Table 1.
Forward and reverse primers used for real-time quantitative reverse transcriptase polymerase chain reaction and their respective Ct values
| Gene of Interest (n) | Primer Sequence | Ct (Means ± SE) |
|---|---|---|
| 18S (4) | Forward: 5′-CAGAAGGACGTGAAGGATGG-3′ | 16.624 ± 0.359 |
| Reverse: 5′-CAGTGGTCTTGGTGTGCTGA-3′ | ||
| SRY (2) | Forward: 5′-AAGCGCCCCATGAATGCATTTATGGT-3′ | 29.095 ± 1.431 |
| Reverse: 5′-ACACTTTAGCCCTCCGATGAGGCTGA-3′ | ||
| CRF (4) | Forward: 5′-GGAGCCGCCCATCTCTCT-3′ | 19.036 ± 0.063 |
| Reverse: 5′-TCCTGTTGCTGTGAGCTTGCT-3′ | ||
| AVPhnRNA (4) | Forward: 5′-GCCCTCACCTCTGCCTGCTA-3′ | 22.49 ± 0.211 |
| Reverse: 5′-CCTGAACGGACCACAGTGGT-3′ | ||
| TRPV4 (4) | Forward: 5′-ACAGCAACCTGGAGACTGTGCTTA-3′ | 23.01 ± 0.145 |
| Reverse: 5′-AGTCCTTGAACTTGCGAGACAGGT-3′ | ||
| AT1aR (5) | Forward: 5′-CAAAAGGAGATGGGAGGTCA-3′ | 22.488 ± 0.732 |
| Reverse: 5′-AGCAGTTTGGCTTTGCAACT-3′ | ||
| AT1aR (3) | Forward: 5′-AGCCTGCGTCTTGTTTTGAG-3′ | 27.52 ± 0.825 |
| Reverse: 5′-GCTGCCCTGGCTTCTGTC-3′ | ||
| AT1bR (3) | Forward: 5′-AGAAGAACACGCCAAGAA-3′ | 24.997 ± 0.738 |
| Reverse: 5′-TGAATGAGCACATCCAGAA-3′ |
Numbers of independent experiments are represented in parenthesis. Comparative threshold (Ct) data are represented as means ± SE. 18S, ribosomal mRNA; CRF, corticotropin releasing factor; AVP hnRNA, arginine vasopressin heteronuclear RNA; AT1aR, ANG II type 1a receptor; AT1bR, ANG II type 1b receptor.