Fig. 1.

mHypoE-37 neurons exhibit robust circadian rhythms of core clock genes. The mHypoE-37 neurons were placed in serum-free media for 12 h prior to receiving a 30% serum shock. Following synchronization, total RNA was harvested every 3 h for 36 h. RNA was subsequently converted into cDNA and used for qRT-PCR with Bmal1 (i), Per2 (ii), Rev-erbα (iii), and Clock (iv)-specific primers. All mRNA values are normalized to Histone 3a expression. A: Bmal1, Per2, and Rev-erbα mRNA levels in the mHypoE-37 cell line are expressed in circadian manner with a period length of 23.73 ± 0.72 h, 22.88 ± 1.21 h, and 28.28 ± 1.80 h, respectively. Transcription of Clock is rhythmic, but not circadian. B: Superimposition of Bmal1-Per2 (left) and Bmal1-Rev-erbα (right) expression profiles reveals that the genes are cycling in opposing phases. Values are plotted as mean values ± SE of three individual experiments.