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Zymographic analysis of phosphorylation of Akt and ERK1/2 in vivo. A and B: Western blots of total and phosphorylated (phospho) ERK1/2. Whole-lung homogenates were loaded for WT and uPA-deficient mice. These experiments were repeated 3 times. Densitometric ratio of phosphorylated/total proteins for ERK1/2 was computed. *P < 0.05. N = 9. C and D: Western blots of total and phosphorylated Akt. Densitometric ratio of phosphorylated/total proteins for Akt (D). N = 12.
