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. 2014 Aug 29;307(8):L609–L617. doi: 10.1152/ajplung.00126.2014

Fig. 5.

Fig. 5.

Immunoblot analysis of phosphorylation of Akt and ERK1/2 in vitro. A and B: Western blots of total and phosphorylated ERK1/2. Lysates were prepared from WT and uPA-deficient primary cultured MTE cells. The cells were mounted on Ussing chambers for functional studies prior to homogenization. Densitometric ratio of phosphorylated/total proteins for ERK1/2. *P < 0.05. N = 12. C and D: Western blots of total and phosphorylated Akt. Densitometric ratio of phosphorylated/total proteins for Akt (D). N = 12. E: amiloride-sensitive Isc in cells post-ERK1/2 knockdown. Scrambled siRNAs (scRNA) were transfected in WT and uPA-deficient controls. N = 5, *P < 0.05 vs. WT and &P < 0.05 vs. uPA−/− cells.