TABLE I.
Comparison of Hematologic Markers and Measures of Iron Stores in Hepc1 (−/−) and WT Mice After 3 weeks of Sterile Abscesses
| Parameter | WT C57BL/6 |
Hepc1 (−/−) |
Genotype × treatment Hepc1 (−/−) versus WT |
IL-6 (−/−) |
Genotype × treatment IL-6 (−/−) versus WT |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Control (N≥14) Mean ± SD |
3w Abscess (N = 10) Mean ± SD |
P valuea | Control (N ≥6) Mean ± SD |
3w Abscess (N ≥6) Mean ± SD |
P valuea | P valueb | Control (N ≥9) Mean ± SD |
3w Abscess (N = 10) Mean ± SD |
P value a | P value b | |
| Neutrophil (K/mcL) | 0.30 ± 0.14 | 0.86 ± 0.41 | 0.002 | 0.41 ± 0.32 | 1.10 ±1.02 | 0.024 | 0.684 | 0.44 ± 0.21 | 1.23 ± 0.29 | <0.001 | 0.150 |
| Monocyte (K/mcL) | 0.14 ± 0.12 | 0.36 ± 0.23 | 0.016 | 0.86±1.24c | 0.99 ± 0.96 | 0.760 | 0.835 | 0.08 ± 0.04 | 0.38 ± 0.12 | <0.001 | 0.332 |
| RBC (M/mcL) | 9.58 ± 0.45 | 8.72 ± 0.45 | <0.001 | 9.90 ± 0.44 | 9.14 ± 0.51 | <0.001 | 0.691 | 9.59 ± 0.24 | 8.79 ± 0.38 | <0.001 | 0.794 |
| Hgb (g/dL) | 14.1 ± 0.8 | 13.0 ± 0.7 | 0.001 | 15.0 ± 0.6 c | 14.9 ± 0.64 | 0.399 | 0.022 | 13.9 ± 0.5 | 13.3 ± 0.3 | 0.016 | 0.190 |
| MCV (fL) | 46.9 ± 2.0 | 46.1 ±1.4 | 0.191 | 50.8 ± 3.5 c | 54.3 ± 3.3 | 0.011 | 0.004 | 46.7 ± 0.9 | 48.5 ±1.9 | 0.017 | 0.013 |
| MCH (pg) | 14.7 ±0.4 | 14.9 ± 0.3 | 0.118 | 15.2 ± 0.4 c | 16.3 ±0.7 | <0.001 | 0.002 | 14.4 ± 0.3 | 15.2 ± 0.5 | 0.001 | 0.041 |
| RDW (%) | 17.0 ±0.6 | 19.2 ± 0.7 | <0.001 | 16.9 ±0.8 | 18.3 ± 0.9 | <0.001 | 0.053 | 16.7 ±0.5 | 19.3 ±1.1 | <0.001 | 0.339 |
| Platelets (K/mcL) | 774 ± 75 | 1,191 ±107 | <0.001 | 749 ± 148 | 1,067 ±187 | <0.001 | 0.175 | 743 ± 47 | 847 ±123 | 0.028 | <0.001 |
| Retics (×109/L) | 269 ± 83 | 406 ± 82 | <0.001 | 216 ±50 | 273 ± 120 | 0.112 | 0.113 | 215 ±16 | 274 ±106 | 0.112 | 0.119 |
| Retics (%) | 2.82 ±1.02 | 4.68 ±1.02 | <0.001 | 2.19 ± 0.48 | 3.02 ± 1.39 | 0.046 | 0.085 | 2.24 ± 0.17 | 3.14 ±1.25 | 0.050 | 0.115 |
| Mouse (g) | 16.4 ±1.4 | 16.4 ±1.3 | 0.997 | 17.7 ±1.6 | 17.1 ± 0.83 | 0.280 | 0.442 | 15.2 ±1.3 | 16.2 ± 0.8 | 0.049 | 0.210 |
| Spleen (mg) | 55.4 ±11.8 | 133.4 ± 36.7 | <0.001 | 68.6 ± 12.0 c | 132.2 ± 23.9 | <0.001 | 0.215 | 41.6 ± 4.8 c | 104.5 ±28.9 | <0.001 | 0.230 |
| Marrow (% Ter) | 62.9 ± 4.9 | 30.6 ±11.1 | <0.001 | 61.4 ±8.0 | 30.4 ± 6.6 | <0.001 | 0.805 | 65.8 ± 3.3 | 20.5 ±6.0 | <0.001 | 0.002 |
| Splenocytes (%Ter) | 37.4 ± 3.6 | 58.9 ± 9.3 | <0.001 | 39.7 ± 6.2 | 57.9 ± 2.7 | <0.001 | 0.450 | 44.8 ± 14.7 | 60.4 ± 7.8 | 0.015 | 0.305 |
| Liver Fe (mcg/g) | 129.4 ± 52.4 | 118.5 ±26.6 | 0.480 | 2,020 ± 497 c | 1,150 ± 339 | 0.006 | <0.001 | 127.6 ± 23.7 | 139.4 ±21.2 | 0.223 | 0.288 |
| Fe per spleen (mcg) | 32.15 ± 14.57 | 105.0 ±40.5 | <0.001 | 3.56 ± 2.61 c | 14.26 ± 5.26 | 0.003 | <0.001 | 32.58 ± 13.64 | 46.41 ± 14.54 | 0.034 | <0.001 |
a
Student’s t-test, unequal variance.
b
Two-way factorial ANOVA.
c
P <0.05 for comparison of either untreated Hepc1 (−/−) or untreated IL-6 (−/−) versus untreated WT. After one-way ANOVA comparing control groups of WT, Hepc1(−/−), and IL-6 (−/−) mice, pairwise tests were based on Scheffe’s multiple comparison test.