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. 2014 Oct 17;4:6666. doi: 10.1038/srep06666

Figure 4. Effects of intracellular NAD on SIRT1 expression and activity.

Figure 4

(a–d), Relative NAD levels, SIRT1 activity, and SIRT1 mRNA and protein levels after incubation with different concentrations of NAD in A2780 cells (repeated 12 times) and primary non-BRCA1-mutated and BRCA1-mutated ovarian cancer cells (n = 12). 1–5: incubation with 0, 5, 25, 125, or 625 μM NAD. Bar graphs show mean ± SD. * P < 0.05 vs. control. (e–h), Relative NAD levels, SIRT1 activity, and SIRT1 mRNA and protein levels after knockdown or overexpression of Nampt in A2780 cells (repeated 12 times) and primary non-BRCA1-mutated and BRCA1-mutated ovarian cancer cells (n = 12). Bar graphs show mean ± SD. Sh, shRNAs; Op, overexpression. * P < 0.05 vs. control.