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. 2014 Oct 17;4:6640. doi: 10.1038/srep06640

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(a) HPLC analysis of tyrosol (3) production in the fermentation supernatant of recombinant strains. 1. BMGF1, E. coli MG1655 (ΔfeaB) harboring plasmid pTrc1 (P_Trc-ARO10); 2. BMGF0, E. coli MG1655 (ΔfeaB) harboring empty vector pTrcHisB (control); 3. Standard tyrosol (3). (b) Mass spectrum of tyrosol (3) from fermentation supernatant of strain BMGF1. (c) The influence of gene deletions on the tyrosol production. All the strains derived from BMGF1. The genes pykA, tyrR, pykF and pheA were sequentially deleted to enhance the metabolic flux toward 4-hydroxyphenylpyruvate (2), resulting strains BMGP1, BMGT1, BMGK1 and BMGA1. (d) The influence of genes overexpression on the tyrosol production. BMGA2, BMGA3, BMGA4 and BMGA5 were derived from BMGA1 harboring empty vector pBb0 (as control), pBb1 (P_LtetO−1-tyrA*^syn-aroG*^syn-ppsA), pBb2 (P_LtetO−1-tyrA*^syn-aroG*^syn-ppsA and P_lacUV5-tktA-aroE) and pBb3 (P_LtetO−1-tyrA*^syn-aroG*^syn-ppsA, P_lacUV5-tktA-aroE and P_lacUV5-aroD-aroB^op), respectively. Three replicates were performed, and the error bars represented standard deviation.