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. 2014 Oct 17;4:6653. doi: 10.1038/srep06653

Table 1. Primers used for mucin gene expression analysis.

Mucin transcript RT-PCR Tissue RT-PCR and in situ hybridization
muc5.1 muc5.1
F1- IJ1 – tgacatgggttgaaagcaaa F – IJ7- tgttccaggcacacattgat
R1 – IJ4 – tggtggtgttgaaaatgtga R – IJ28 - cgacaaatcgtgtgagaaca
F3 - IJ7 – catccctaggcacatccact muc5.2
R3 - IJ63 – tgttttgcgtcgcttaagaa F – IJ68 - ctgagatgggtcatcctgct
muc5.2 R – IJ31 - ggtactgctggcaaaccatt
F1 - IJ68 – ctgagatgggtcatcctgct muc5.3
R1 - IJ71 – gtctcgggtgatgaaggtgt F – IJ80 - cgagcaatatgcacagcact
F2 - IJ50 – cagtgcatcgagaccaacag R – IJ81 - gctcgagcagttgaaaaacc
R2 - IJ51 – tcatcattcgcctaattcca muc2.1
muc5.3 F – IJ125 - tgctgcttctggcgctttctggt
F1 - IJ80 – cgagcaatatgcacagcact R – IJ126 - ttgctgtcctccatgcgggtg
R1 - IJ81 – gctcgagcagttgaaaaacc muc2.2
F2 - IJ82 – agatgcctgctgtccagagt F – IJ167 - accacaaccaaacccatgtt
R2 - IJ83 – aggttccacacaaccctgag R – IJ168 - gttccacattggccttctgt
muc2.1  
F1 – IJ125 – tgctgcttctggcgctttctggt  
R1 - GY2 – cataaccaatttccccatcg Muc5.1 primers for in situ hybridization
muc2.2 muc5.1
F1 - IJ111 – actgtccgtgtcctcatggt F – IJ1 - tgacatgggttgaaagcaaa
R1 - IJ112 – tctcctccagtgtcacatgc R – IJ3 - aaccgtgaccgtttcttcac
F2 - IJ113 – accacaaccaaacccatgtt  
R2 - IJ114 – cctctcgaatgctggatctc  
qRT-PCR  
muc5.1 muc2.1
F – tggcaacttggctgatgata F – GY3 - aatatgccttgcggaacaac
R – tcgtcacacggaccagtaga R – GY4 - gtgctgaggttgcagaatga
muc5.2 beta-actin2
F – ggtgtctgttccgatcaatc F - cgtgctgtcttcccatcca
R – tcatccttgtcgccattgta R – tcaccaacgtagctgtctttctg
muc5.3  
F – GY5 - ggggaaaactacaccagcaa  
R – GY6 - tgtgaattctgtgccagagc