Figure 4. Asymmetric lipid dequenching is seen with different pairs of fluorescent lipidic markers.
(A–C) Lipid dequenching reactions of 1R- and 3Q-RPLs (250 µM lipid each) had combinations of Sec17p (600 nM), Sec18p (200 nM), and HOPS (100 nM), as indicated. Either the 1R-RPLs (light gray) or the 3Q-RPLs (dark gray) bore pairs of fluorescently labeled lipids (1.5 mol% each): Marina-Blue (MB) and NBD (A), NBD and rhodamine (Rh) (B), or Oregon-Green (OG) and Texas-Red (TR) (C).
Figure 4—figure supplement 1. Representative kinetic data for panels A–C in figure 4.
