Table 1.
Number of mtDNA sequence haplotypes in the single CD34+ cell population
| Donor1 | Sample2 | Age/Sex | Live cells / Gated cells (%)3 |
CD34+ cells / Live cells (%)3 |
No. of cells for mtDNA analysis |
No. of haplotypes |
No. of haplotypes defined by nucleotide substitutions |
No. of haplotypes / 100 cells |
No. of haplotypes by substitutions / 100 cells |
|---|---|---|---|---|---|---|---|---|---|
| Donor #1 | Sample 5 | 51/M | 62.55 | 0.208 | 96 | 10 | 5 | 10.4 | 5.2 |
| B-8 | 91.92 | 0.371 | 94 | 35 | 28 | 37.2 | 29.8 | ||
| Donor #2 | Sample 10 | 89/F | 70.29 | 0.079 | 95 | 22 | 14 | 23.2 | 14.7 |
| B-3 | 96.41 | 0.026 | 86 | 48 | 42 | 55.8 | 48.8 | ||
| Donor #3 | Sample 6 | 60/F | 77.95 | 0.099 | 95 | 19 | 10 | 20.0 | 10.5 |
| B-6 | 89.71 | 0.025 | 95 | 47 | 38 | 49.5 | 40.0 | ||
| Donor #4 | Sample 9 | 87/F | 71.24 | 0.019 | 95 | 13 | 8 | 13.7 | 8.4 |
| B-5 | 87.69 | 0.025 | 95 | 49 | 40 | 51.6 | 42.1 |
1
These four donors were maternally related and belonged to family B described in our recent study [15]. Donors #1, #2, #3, and #4 refer to family members B-8, B-3, B-6 and B-5, respectively.
2
Samples from the same donors that were included in group I and group II were represented marked by “Sample” and “B-”, respectively. The mtDNA sequence data for samples of group II (B-8, B-3, B-6, and B-5) were taken from Yao et al. [15].
3
The gating for living cells and CD34+ cells was defined in Figure 1.