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Cultured Schwann cells at passages 2–3 (inverted microscope, × 40).
Cultured Schwann cells were treated in duplicate consistently with 5.6 mM of glucose as a control, consistently with 50 mM of glucose as high glucose, with high glucose in the presence of 1, 10 and 100 μM of puerarin for 48 hours. In addition, the cells were treated with 44.4 mM of mannitol (Sigma, St Louis, MO, USA) plus 5.6 mM glucose for osmotic controls.
