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. 2012 Dec 5;7(34):2705–2712. doi: 10.3969/j.issn.1673-5374.2012.34.008

Figure 1.

Figure 1

Neurite outgrowth induced by Rho-associated kinase (ROCK) inhibitors in PC12 Adh cells (A, B) and Neuro-2a cells (C, D).

Cells with longer neurite(s) than their soma were defined as neurite positive cells. The neurite positive cell rate (%) was calculated as positive cells/total cells × 100%.

Neurite outgrowth in PC12 Adh cells following continuous exposure to ROCK inhibitors (33 μM Y27632 or 33 μM fasudil) decreased by the third day of treatment as shown in optical microscope images (A) and graphically (B).

Neurite outgrowth in Neuro-2a cells following continuous exposure to ROCK inhibitors (33 μM Y27632 or 33 μM fasudil) persisted beyond the third day of treatment as shown in optical microscope images (C) and graphically (D). NGF (1.0 μg/mL) induced neurite outgrowth in PC12 Adh cells (A and B) but not in Neuro-2a cells (C and D).

Scale bars in A, C: 100 μm. Data in Figures B and D are expressed as mean ± SD. aP < 0.05, vs. 0 day (one-way analysis of variance, n = 3). NGF: Nerve growth factor.