Figure 4.

(A) Purification of MsV1H and identification by western blotting. WB: western blotting; M: protein marker; E₁: 1st elution; E₂: 2nd elution; L: total cell lysate; F: flow-through elution; (B) Protein score got from mass spectrometry. Protein score reflects the combined scores of all observed mass spectra that can be matched to amino acid sequences within that protein. A higher score indicates a more confident match. The number of protein matches at each scoring position is indicated by the height of the red bars. The non-significant area is shaded in green. Most matches are non-significant. However, one protein match is strongly significant; it is B. mori V-ATPase subunit H with protein score 129; (C) Summary of top ranking protein. The score value listed within the query table is the Ion Score. The Ion Score is a measure of how well the observed MS/MS spectrum matches to the stated peptide. The confidence threshold for Ion Scores is the same as that for the Protein Score. Ions Scores >28 were statistically significant (p < 0.05). The expected value indicates the probability that the observed match between MS/MS spectra and peptide sequence would be found by chance. Confident matches typically have expected values <0.1.