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. 2014 Sep 23;15(9):16885–16910. doi: 10.3390/ijms150916885

Figure 4.

Figure 4

(A) SDS-PAGE. Lane 1: protein molecular weight markers; Lane 2: the soluble fraction of the cell lysate after induction; Lane 3: the insoluble fraction of the cell lysate after induction; Lane 4: fluid through Ni-NTA column; Lane 5: purified fraction from Ni-NTA eluted by washing buffer containing 30 mM imidazole; Lane 6: purified fraction from Ni-NTA eluted by washing buffer containing 60 mM imidazole; Lane 7: purified fraction from Ni-NTA eluted by washing buffer containing 100 mM imidazole; Lane 8: purified rP186_1588 eluted by washing buffer containing 200 mM imidazole; (B) Western blotting analysis. Lane 1: protein markers; Lane 2: purified rP186_1588; (C) Activity testing on tributyrin plate. Lane 1: rP186_1588; Lane 2: buffer solution (NTA-0).