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Kinetic parameters for P186_1588 with p-NP esters.
| Substrate | Km (mM) | kcat (s−1) | kcat/Km (s−1·mM−1) | Specific Activity (U/mg) |
|---|---|---|---|---|
| p-NP acetate (C2) | 0.35 ± 0.01 | 11.65 ± 0.48 | 33.29 ± 1.37 | 33.07 ± 1.36 |
| p-NP butyrate (C4) | 1.27 ± 0.04 | 9.12 ± 0.62 | 7.18 ± 0.19 | 25.90 ± 1.76 |
| p-NP caprylate (C8) | 2.64 ± 0.14 | 6.36 ± 0.28 | 2.41 ± 0.11 | 18.05 ± 0.79 |
| p-NP decanoate (C10) | 4.99 ± 0.21 | 6.19 ± 0.33 | 1.24 ± 0.07 | 17.57 ± 0.94 |
* Values are means ±SE from three independent experiments.
