Table 1.
Chemical composition of used growth media.
| Stem Cell Growth Medium | Concentration |
|---|---|
| Selenium (Sigma–Aldrich, Munich, Germany) | 5 ng/mL |
| Transferrin (Sigma–Aldrich) | 5 µg/mL |
| Linoleic acid (Sigma–Aldrich) | 4.7 µg/mL |
| Insulin (Sigma–Aldrich) | 5 µg/mL |
| Ascorbic acid (Sigma–Aldrich) | 1 µg/mL |
| Dexamethasone (Sigma–Aldrich) | 1 µg/mL |
| MCDB 201 with l-glutamine solution (Sigma–Aldrich) | 34 mL/100 mL |
| Dulbecco’s modified Eagle’s medium (Biochrom AG) | 51 mL/100 mL |
| Fetal calf serum (FCS, Biochrom AG) | 15 mL/100 mL |
| Streptomycin (Biochrom AG) | 50 IU/mL |
| Penicillin (Biochrom AG) | 50 IU/mL |
| Chondrocytes Growth Medium | Concentration |
| Ham’s F-12/Dulbecco’s modified Eagle’s medium supplemented with 25 μg/mL ascorbic acid (Sigma–Aldrich) | 1000 mL |
| Streptomycin (Biochrom AG) | 50 IU/mL |
| Penicillin (Biochrom AG) | 50 IU/mL |
| Amphotericin B (Biochrom AG) | 2.5 μg/mL |
| Essential amino acids (Biochrom AG) | 1 mL/100 mL |
| Fetal calf serum (Biochrom AG) | 1 mL/100 mL |
| Lipogenic Medium | Concentration |
| Indomethacin (Sigma–Aldrich) | 2 µL/mL |
| Isobutyl-1-methylxanthine (Sigma–Aldrich) | 1 µL/mL |
| Rosiglitazone (Sigma–Aldrich) | 1 µL/mL |
| Insulin (Sigma–Aldrich) | 4 µL/mL |
| Dexamethasone (Sigma–Aldrich) | 1 µL/mL |
| Fetal calf serum (FCS, Biochrom AG) | 0.1 mL/mL |
| Streptomycin (Biochrom AG) | 50 IU/mL |
| Penicillin (Biochrom AG) | 50 IU/mL |
| HEPES (Biochrom AG) | 25 µL/mL |
| Dulbecco’s modified Eagle’s medium with 3.7 g/L NaHCO3 and 4.5 g/L glucose (Biochrom AG) | 10 mL |
| Osteogenic Medium | Concentration |
| Dexamethasone (Sigma–Aldrich) | 1 µL/mL |
| Glycerol-3-phosphate (Sigma–Aldrich) | 10 µL/mL |
| Ascorbic acid (Sigma–Aldrich) | 2 µL/mL |
| HEPES (Biochrom AG) | 25 µL/mL |
| Streptomycin (Biochrom AG) | 50 IU/mL |
| Penicillin (Biochrom AG) | 50 IU/mL |
| Chondrogenic Medium | Concentration |
| l-Glutamine (Biochrom AG) | 10 µL/mL |
| HEPES (Biochrom AG) | 25 µL/mL |
| Sodium pyruvate (Sigma–Aldrich) | 10 µL/mL |
| Dexamethason (Sigma–Aldrich) | 0.1 µL/mL |
| Ascorbic acid (Sigma–Aldrich) | 1.7 µL/mL |
| Prolin (Sigma–Aldrich) | 1 µL/mL |
| ITS+1 (Sigma–Aldrich) | 10 µL/mL |
| Streptomycin (Biochrom AG) | 50 IU/mL |
| Penicillin (Biochrom AG) | 50 IU/mL |
| TGF-β1 (Pepro Tech GmbH, Hamburg, Germany) | 10 ng/mL |
| Dulbecco’s modified Eagle’s medium with 3.7 g/L NaHCO3 and 4.5 g/L glucose (Biochrom AG) | 10 mL |