Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sequences of the primers used in the present study.
| Gene (Symbol) | NCBI Gene Reference | Length | Manufacturer |
|---|---|---|---|
| β-actin (ACTB) | NM_001101.2 | 171 | ABI® * |
| aggrecan (ACAN) | NM_013227.2 | 93 | ABI® * |
| sox9 (SOX9) | NM_000346.2 | 102 | ABI® * |
| TNFα (TNFα) | NM_000594.2 | 80 | ABI® * |
| β-actin (ACTB) (5'–3') | TGGGACGACATGGAGAA/GAAGGTCTCAAACATGATCTGG | 146 | Qiagen® |
| type II collagen (COL2A1) | NM_001844, NM_033150 | 142 | Qiagen® |
* ABI, Applied Biosystems® (Life Technologies™, Carlsbad, CA, USA).
