Expression patterns of CsCTR1 during different flower developmental stages. (A) Morphology of male flowers (MF) and female flowers (FF) at five developmental stages, which were separated on the basis of the corolla length. MF1/FF1 = (2.5 ± 1) mm, MF2/FF2 = (5 ± 1) mm, MF3/FF3=(9 ± 2) mm, MF4/FF4=(15 ± 2) mm, MF5/FF5=(20 ± 2) mm; (B) Relative expression of CsCTR1 normalized to the cucumber Actin3 gene used as internal control in female flowers and male flowers at different developmental stages. Each result was the average of three independent biological replicates. Error bars on each column indicate standard deviation. The statistical significance was determined by Duncan’s multiple comparison tests and Student’s t-test. Different letters above bars indicate significant differences (p < 0.05). The same letter indicates no significant difference (p < 0.05). * p < 0.05, ** p < 0.01 by the Student’s t-test.