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. 2014 Aug 29;15(9):15287–15303. doi: 10.3390/ijms150915287

Scheme 1.

Scheme 1

Schematic illustration of formation of HA/FA/PPD (hyaluronic acid (HA) and folate (FA)-modified liposomes). Firstly, PEI (polyethylenimine) bind DNA to form the condensed cationic PEI/DNA complexes, which were chosen as the cationic core of the liposomes. Subsequently, PTX (paclitaxel) and PEI/DNA complexes were co-loaded in the DSPE-PEG2000-FA-modified liposomes, forming the FA-modified cationic liposomes (FA/PPD). Lastly, the cationic FA/PPD was added to the anionic HA solution to obtain the HA coated FA/PPD (HA/FA/PPD) by electrostatic attraction.