Figure 1.

Experimental design and workflow using the permanent middle cerebral artery occlusion (PMCAO) model. Effect of the intracerebroventricular administered pituitary adenylate-cyclase activating polypeptide 38 (PACAP38) into ischemic mouse brain was evaluated at the molecular level in the ischemic core and penumbra of the ipsilateral (right) hemisphere along with the corresponding regions in the healthy contralateral hemisphere (left). All marked brain regions were sampled and finely powdered in liquid nitrogen, followed by investigation into molecular level changes at the level of genes (mRNA abundance) and protein expression by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively.