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Detection of active c-Src in GD25-β1A and GD25-β1AK756L cells adhering to anti-β1 mAb. c-Src was immunoprecipitated from cell lysates prepared from suspended cells (S) or from cells adhering to anti-β1 mAb for 1 h. The immunoprecipitates were resolved by SDS-PAGE and transferred to nitrocellulose filters. The membranes were first blotted for pY-416 Src and, after stripping, for total c-Src.
