Figure 2.

Transfection efficiencies of hAD-MSCs and fibroblasts using four independent techniques. The microporation technique had the highest transfection efficiency as compared to standard electroporation and chemical-based transfection reagents for transfection of (A) hAD-MSCs and (B) fibroblasts. hAD-MSCs and fibroblasts were transfected with a plasmid encoding for Angiopoietin-1 (ANGPT-1) and the enhanced green fluorescent protein (eGFP) expression cassette using different chemical-based reagents and electroporation protocols after optimization.