Figure 4.
Post-transfection proliferative potential of hAD-MSCs and fibroblasts. Analysis of (A) hAD-MSC and (B) fibroblast proliferation capability following transfection using microporation, standard electroporation, cationic polymer, and classical calcium phosphate precipitation transfection techniques. Two days post-transfection, the cells were trypsinized and seeded at 3 × 103 cells/well in 96-well plates. The Trypan blue exclusion assay was used to determine total cell number in the hAD-MSC and fibroblast cultures at days 3, 5, and 7 after plating. hAD-MSCs and fibroblasts transfected using the microporation, standard electroporation, and classical calcium phosphate precipitation techniques exhibited a greater proliferation potential comparable to non-transfected cells. However, transfection with the cationic polymer repressed the hAD-MSCs and fibroblasts proliferation.