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. 2014 Aug 28;15(9):15173–15187. doi: 10.3390/ijms150915173

Figure 2.

Figure 2

Curcumin promoted proteasome-dependent degradation of KLF5 protein. (A) 5637 and WH cells were pretreated with 10 μM MG132 for one hour before the treatment of curcumin. Twelve hours later, the whole-cell lysates were harvested, and KLF5 protein was analyzed by western blot; (B) KLF5 protein attenuation in the cycloheximide (CHX) chase assay was examined after DMSO or curcumin treatment in 5637 cells; (C) KLF5 protein levels were quantified using Image Lab software and were normalized to GAPDH; and (D) KLF5 half-life values were calculated by nonlinear regression analysis with GraphPad Prism software. The results represent three independent experiments with similar results. Error bars show the 95% confidence intervals. * p < 0.05.