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. 2014 Aug 28;15(9):15173–15187. doi: 10.3390/ijms150915173

Figure 3.

Figure 3

KLF5 mediated the anti-proliferative effect of curcumin. (A,B) KLF5 and cyclin D1 expression in curcumin-treated 5637 and WH cells (A) and in lentivirus-shKLF5-infected stable cells (B), as detected by western blot analysis; (C) The growth rates of 5637 KOSC and KOKLF5 cells were determined by the MTT assay; (D) Flag-KLF5 or vector-transfected 5637 cells were treated with 15 μM curcumin for 24 h. Then, cell lysates were harvested, and the KLF5 and cyclin D1 levels were analyzed by the western blot assay;and (E) The cell viability of different groups in (D) was determined by the MTT assay. The results represent three independent experiments. The values are the mean ± SD. * p < 0.05.