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. 2014 Sep 9;15(9):15845–15857. doi: 10.3390/ijms150915845

Figure 1.

Figure 1

Over-expression of α-Syn in SH-SY5Ycell line. (A) Relative mRNA level of α-Syn in the SH-SY5Y-Syn(+) cells post various passages, compared to β-actin, revealedby quantitative real-time RT-PCR; (B) Western blot analysis of α-Syn, LAMP2A and Hsc 70 in the SH-SY5Y-Syn(+) cells, post various passages;(C) Significantly high level of α-Syn in variously propagated SH-SY5Y-Syn(+) cells; (D) LAMP2A and Hsc 70 protein level in the SH-SY5Y-Syn(+) cells, post various passages. Data represent the mean ±SEM of three independent experiments. Statistical significance was considered with a p-value <0.05 or less, * p < 0.05, ** p < 0.01, ns, no significance.