Table 1.
Primer design for quantitative RT-PCR analysis.
| mRNA | Primers | Annealing Tm (Cycle) |
|---|---|---|
| SOX9 | Fw: 5'-ATCTGAAGAAGGAGAGCGAG-3' | 58 °C (32) |
| Rv: 5'-TCAGAAGTCTCCAGAGCTTG-3' | ||
| Col2α1 | Fw: 5'-AACACTGCAACGTCCAGAT-3' | 58 °C (32) |
| Rv: 5'-CTGCAGCACGGTATAGGTGA-3' | ||
| Cartilage link protein | Fw: 5'-GCGTCCGCTACCCCATCTCTA-3' | 55 °C (32) |
| Rv: 5'-CTCTAAGGGCACATTCACTT-3' | ||
| Aggrecan | Fw: 5'-GAGGTCGTGGTGAAAGGTGT-3' | 58 °C (32) |
| Rv: 5'-GTGTGGATGGGGTACCTGAC-3' | ||
| ADAMS5 | Fw: 5'-TGTCCTGCCAGCGGATGT-3' | 58 °C (30) |
| Rv: 5'-ACGGAATTACTGTACGGCCTACA-3' | ||
| MMP-1 | Fw: 5'-TCAGTTCGTCCTCACTCCAG-3' | 58 °C (30) |
| Rv: 5'-TTGGTCCACCTGTCATCTTC-3' | ||
| MMP-13 | Fw: 5'-GATAAAGACTATCCGAGAC-3' | 58 °C (30) |
| Rv: 5'-CGAACAATACGGTTACTC-3' | ||
| TGF-β | Fw: 5'-CATCTGGAGCCTGGATACACAGT-3' | 56 °C (30) |
| Rv: 5'-GAAGCGCCCGGGTTGT-3' | ||
| BMP-2 | Fw: 5'-CGGGAACAGATACAGGAAGC-3' | 60 °C (30) |
| Rv: 5'-GCTGTTTGTGTTTGGCTTGA-3' | ||
| BMP-4 | Fw: 5'-GAGTATCTAGCTTGTCTCCCC-3' | 56 °C (30) |
| Rv: 5'-TCAGGTATCAAACTAGCATGG-3' | ||
| β-actin | Fw: 5'-GCTCTCCAGAACATCACTCCTGCC-3' | 58 °C (30) |
| Rv: 5'-CGTTGTCATACCAGGAAATGAGCTT-3' |
Fw, forward; Rv, reverse; Tm, temperature.