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. 2004 Jun;15(6):2627–2638. doi: 10.1091/mbc.E03-09-0688

Figure 2.

Figure 2.

Heterologous expression of RyR2 TM domains in G7 and E6 cells. (A) Microsomal fractions (100 μg) obtained from G7 and E6 cells after ponA induction (0-20 μM) of RyR24TM and RyR210TM, respectively, were immunoblotted using pAb129. (B) Endogenous eGFP fluorescence was used to visualize RyR24TM and RyR210TM in G7 and E6 cells, respectively, after induction with ponA (0-20 μM). Bar, 10 μm. (C) Nuclear (N), microsomal (M), and cytosolic (C) fractions (100 μg) obtained from CHORxR, G7, and E6 cells exposed to ponA (5 μM) were immunoblotted using pAb129. (D) Intracellular localization of RyR2 TM domains in G7 and E6 cells after induction with ponA (5 μM). Bar, 5 μm.