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Secretion of ET-1 by normal and SSc lung fibroblasts. The concentration of ET-1 in conditioned media was measured by an ELISA that detects ET-1 levels. Medium conditioned for 24 h by normal and SSc lung fibroblasts (SScLF) was examined. Data shown are means (+SEM) based on five replicate wells for three independent experiments by using different fibroblast strains (*p < 0.05, Student's unpaired t-test).
