Table 2.

Effect of the decrease of AKAP350 expression on the Golgi apparatus

	siRNA control	siRNA1	siRNA2
p58
n	34	31	29
Golgi (% area per cell)	9.7 ± 0.5	15.2 ± 1.3*	16.9 ± 3.0*
Golgi I/total I	2.7 ± 0.2	1.6 ± 0.1**	1.6 ± 0.1**
golgin-97
n	48	52	25
Golgi (% area per cell)	7.9 ± 0.8	17.9 ± 1.4**	12.1 ± 0.9*#
Golgi I/total I	3.8 ± 0.2	2.2 ± 0.1**	2.9 ± 0.1**#

The Golgi and total cell areas as well as the average fluorescence intensity (I) for p58 and golgin-97 were measured in HeLa cells transfected for 72 h with the RNA duplexes targeted to specific sequences of AKAP350 (siRNA1 and siRNA2) or with the siRNA control. The decrease in AKAP350 levels induced dispersal of the Golgi apparatus, as stained with p58 and golgin-97 (Figure 6). The results are presented as the means ± SEM of the indicated number of cells analyzed (n).

^*p < 0.05

^**p < 0.01 compared with the controls

^#p < 0.05 compared with siRNA1