Skip to main content
. 2004 Jun;15(6):2782–2793. doi: 10.1091/mbc.E04-03-0179

Figure 3.

Figure 3.

RNAi of pat-10 troponin C results in ovulation defects. (a-c) Western blot analysis of PAT-10 (a), CeTM (b), and actin (c) after control RNAi with a blank vector (lane 1), CeTMI,II (RNAi) (lane 2), CeTMI,II,III,IV (RNAi) (lane 3), or pat-10 (RNAi) (lane 4). PAT-10 was significantly reduced by pat-10 (RNAi) (a, compare lanes 1 and 4) and slightly reduced by CeTMI,II (RNAi) (a, lane 2), CeTMI,II,III,IV (RNAi) (a, lane 3). In contrast, the level of CeTM was not altered by pat-10 (RNAi) (b, compare lanes 1 and 4). (c-i) Control (d, f, and h) or pat-10 (RNAi) (e, g, and i) worms were stained by DAPI (d and e) and rhodamine-phalloidin (f and g). Merged images of DNA (blue) and F-actin (red) are shown in h and i. Positions of the spermatheca (Sp) are indicated in d and e. Large accumulations of DNA in endomitotic oocytes are indicated by arrows in e. Bar, 50 μm.